In which system can I express the GenPoolTM ORF clones directly?
All GenScript GenPoolTM ORF clones are cloned into GenScript pDream2.1/MCS vector, which is a protein expression vector for both efficient cloning and high-level expression of any target genes. The gene of interest can be expressed directly without any further cloning work in any one of the three major protein expression systems: Bacteria, Insect cells and Mammalian cells. • How can I detect or purify the protein? A: All GenScript GenPoolTM ORF clones are cloned into GenScript pDream2.1/MCS vector with a FLAG Tag fused to the 5-end (or N-terminal of the protein). This allows you to detect or purify the tagged protein using specific and sensitive anti-Flag antibodies (Sigma: F7425). This is especially useful when there is no antibody available for the protein that you are working with. • Can I remove the FLAG Tag if I want the authentic protein? A: Yes. The Flag tag sequence is also the cleavage site of enterokinase (EK, GenScript Catalog No.: Z01003). The cleavage by EK will generate a
