In what bacterial protein expression system has the Overnight Express Autoinduction Systems been tested?
Overnight Express Autoinduction Systems have been tested primarily with the pET protein expression system (we have extensively tested T7lac promoter vectors). However, experiments with other IPTG-inducible promoters (i.e., tac and trc promoters) have also been successful. Based on the overall mechanism of action of the system, Overnight Express should be compatible with any IPTG-inducible promoter used for protein expression.