In the case of adherent cells, do you want the cultured cells attached or trypsinized and sent in tubes?
Cells that grow attached to flask or plate surfaces (adherent cells) should be fixed and transferred to tubes according to Genpathway’s fixation protocol. For this, the formaldehyde is added directly to the media of the cultures without use of trypsin. After termination of fixation using the glycine step, a cell scraper is used to scrape the cells from the surface of the flasks or plates, and the cells are collected into tube(s). The remainder of the fixation protocol should then be followed. If adherent cells have been trypsinized, collected in tubes and frozen without prior fixation (with liquid decanted before freezing), they can be sent as is to Genpathway for processing into chromatin or DNA.
Related Questions
- Since reception is performed in raw cells, 1 cell corresponds to 1 packet. In the case of raw cells, I believe that only this configuration is possible, but does this mean that 155 Mbps reception is difficult with raw cells?
- In the case of adherent cells, do you want the cultured cells attached or trypsinized and sent in tubes?
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