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In the Application Guide you give the pipetting scheme for Taqman, and it lists a 20x Taqman probe. What should the final concentration of the probe be?

April 26, 2017application concentration final guide lists pipetting probe Scheme taqman

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In the Application Guide you give the pipetting scheme for Taqman, and it lists a 20x Taqman probe. What should the final concentration of the probe be?

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Each Taqman probe may have its optimal working concentration. You can determine it by doing some pilot experiments. However according to Applied Biosystems protocol, a 1x or 250 nM final concentration usually gives good results.