In general, what are some of the tips and techniques to know in order to take advantage of the frame averaging and histogram functions?
Number of frames to accumulate for averaging. This is very sample and illumination dependent. Use a number of frames just large enough to reduce unwanted background noise. The number of frames to use depends upon the signal strength of the subject of interest compared to the surrounding background noise. More is usually better, but the trade-off is the time necessary to accumulate a larger number of frames than is required. Expand Contrast function. This function uses a default setting of 25 ( 2.5 standard deviations from the average signal) for the shadow and highlight points. Optimal settings depend on the subject, illumination, and user requirements. Increase the contrast of the acquired image by reducing the shadow and highlight value. Decrease the contrast of the acquired image by increasing the highlight value. Dark current noise can be reduced in the acquired image by reducing the shadow value. Adjust Levels function. This function uses a defualt setting of 5 ( 0.5% of the dark
