If the western-blot analysis shows that the immunoreactive band is different from your predicted molecular weight, does it mean that I did not get the right over-expression lysate for my gene?
The predicted the molecular weight is calculated from the protein primary amino acid composition. There could be some discrepancy from the actual molecular weight, if there were post-translational modifications or known proteolytic activity. In this case, OriGene strongly suggests researchers check related literatures on this particular protein.
Related Questions
- If the western-blot analysis shows that the immunoreactive band is different from your predicted molecular weight, does it mean that I did not get the right over-expression lysate for my gene?
- If the coomassie gel shows a protein of a molecular weight different from the predicted molecular weight, does this mean I received the wrong protein?
- The analysis of my purified thiol modified oligonucleotide shows three different species. Are these impurities?
