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If the same assay can detect both gDNA and mRNA, then the primers do not span exon boundaries?

April 26, 2017assay boundaries detect exon gdna mrna Primers span

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If the same assay can detect both gDNA and mRNA, then the primers do not span exon boundaries?

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Correct, our primers do not span exons. Given clean RNA, one can evaluate genomic DNA copy number variation in a sample and determine the gene expression of the same targets using the same system. Therefore it is possible to reveal a two-dimensional barcode of sample such as tumors relating gene copy number with transcription levels.