If my validation vector experiments show incomplete knockdown, does this mean that my RNAi constructs aren’t effective?
No, not necessarily. This validation system is capable of identifying the most effective construct from a set, but should not be used to exclude any one particular construct. This system is best used to determine the most effective construct as a pre-screening tool. However, we don’t have a sufficient amount of data as yet to indicate that our validation vector system fully correlates with the outcome of using other methods for shRNA/siRNA validation at this time.
Related Questions
- Does OriGene provide any guarantee of knockdown in subsequent experiments when an RNAi construct has been validated using this method?
- If my validation vector experiments show incomplete knockdown, does this mean that my RNAi constructs aren’t effective?
- Can I use QuantiTect Gene Expression Assays (GEAs) for validation of gene knockdown in siRNA experiments?
