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If I use the same amount of cDNA for different volume of PCR reaction, will I get different amount or concentration of PCR products?

April 26, 2017cDNA concentration different PCR products REACTION volume

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If I use the same amount of cDNA for different volume of PCR reaction, will I get different amount or concentration of PCR products?

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Theoretically, the PCR product amount should be the same amount but the concentration should vary depend on the PCR volume. If a very faint band from the first round PCR was obtained, then a sharper band should be obtained with a second round PCR using the first round PCR product as templates.