If I use a lower antibody concentration (0.5 mg/mL in 400 µL) for the Amine-Reactive kit, will additional steps/supplies be required?
Yes, because each spin column provided in the kit can only de-salt a volume of 100 µL, you will either need 4 spin columns (2 are provided) to de-salt the activated antibody solution (400 µL), or you will need to concentrate the antibody solution down to 100 µL and use a single spin column, as detailed in the protocol.
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