If I know the dilution and I know the total volume of solution I need to prepare, how do I figure out the volumes of diluent and substance diluted?
You have 3 of the four values to a proportion. If it is a dilution of 1/10 and you need to make 25 mL. Set up the proportion 1 is to 10 and “X” is to 25. Solve for x by “cross multiplying”. X = 2.5. You need to make 25 mL; therefore measure (25 – 2.5) 22.5 mL and add 2.5 mL of substance to dilute.
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