If bran is introduced into the BOD bottle will it adversely affect the 5-day dissolved oxygen uptake rate?
Yes. The bran is simply a carrier for the microbes. Once the PolySeed is hydrated, the microbes soak off the bran. The bran is not a primary food source. Bran should not be present in your test; therefore it is very important not to draw any bran into your pipette. This is why we highly recommend you decant your PolySeed solution after the solution has been hydrated.