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If a sample is diluted to one half of its original concentration, will the readout be one half of the original reading prior to the dilution?

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If a sample is diluted to one half of its original concentration, will the readout be one half of the original reading prior to the dilution?

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Yes, if this value falls within the linear portion of the standard curve. Since the luminometer’s light measurement/reading is directly proportional to the concentration of what is being measured, the RLUs will change proportionally.

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