I would like to inject into lymphocytes with the TransferMan NK 2. Do you have any recommendations?
Microinjection into lymphocytes is a challenge because of their small diameter of approx. 5 µm. This cell type can be adherent or in suspension. In the case of adherent cell culture they can be injected by users with some experience. If you work with lymphocytes in suspension cell culture, you also have to use capillaries with a very small diameter (e.g. CustomTips II) for holding the lymphocytes.