I tried to confirm the insertion site in a stock and found that it differs from the annotated site. What’s going on here?
If have found the insertion site to be off by about 200 nt, and you are referring to the data provided in Supplementary Table 3 of Thibault, et. al, you should read the following paragraphs to more fully understand the data. Otherwise, please Contact Us us and let us know what the problem is. The keys to reading the data in Supplementary Table 3 are the following: The first two letters refer to the element type. (XP, PB, RB or WH) The second entry is the stock number. (one letter followed by five digits) The third entry indicates the number of flanking sequences are associated with that particular stock. In some cases this represents multiple inserts per stock, in others this may represent erroneously assigned sequence files (more on this later). The fourth entry is the R3 coordinate of the insertion site. The fifth entry is the chromosome arm. U is unknown. The sixth entry is the orientation of the element in the chromosome. Finally, the flanking sequence data completes the set of dat
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