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I transformed a pSM2 clone into a standard E. coli strain but nothing grew – why?

April 26, 2017clone coli standard strain transformed

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I transformed a pSM2 clone into a standard E. coli strain but nothing grew – why?

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Plasmid pSM2c contains the RK6γ conditional origin of replication, and requires pir1 expression by host strain for propagation. Thus, a pir1 strain such as PirPlus (DH10βpir116) E. coli must be used for propagation.