I think the Qiagen MinElute columns may be having problems. How can I fix this?
The Qiagen MinElute columns usually perform consistently well, but they do have a number of attributes that require proper attention for maximum performance: • The suggested elution volume is 20 ul, twice the manufacturer’s recommended volume of 10 ul. This is because proper and complete wetting of the column matrix is essential in maximizing yields. We have found that using the 10 ul volume may reduce yields by up to 50%, depending on the efficiency in which the column matrix was wetted. • Qiagen began recommending in 2004 that the columns be stored at 4oC while not in use. If you have some columns that have been sitting at room temperature for months, we suggest using a fresh batch. • The MinElute column should be carefully washed during the washing step to ensure that there are no traces of Buffer ERC. We haven’t found major problems with trace amounts of Buffer ERC in the performance of the TLAD method, but we do notice that it sometimes interferes with quantification by A260. In t
The Qiagen MinElute columns usually perform consistently well, but they do have a number of attributes that require proper attention for maximum performance: • The suggested elution volume is 20 ul, twice the manufacturer’s recommended volume of 10 ul. This is because proper and complete wetting of the column matrix is essential in maximizing yields. We have found that using the 10 ul volume may reduce yields by up to 50%, depending on the efficiency in which the column matrix was wetted. • Qiagen began recommending in 2004 that the columns be stored at 4oC while not in use. If you have some columns that have been sitting at room temperature for months, we suggest using a fresh batch. • The MinElute column should be carefully washed during the washing step to ensure that there are no traces of Buffer ERC. We haven’t found major problems with trace amounts of Buffer ERC in the performance of the TLAD method, but we do notice that it sometimes interferes with quantification by A260. In t
