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I see a large variation in signal for chemifluorescence across the Optimiser™ plate – what is happening?

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I see a large variation in signal for chemifluorescence across the Optimiser™ plate – what is happening?

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• We recommend that if testing with chemifluorescence substrate; readings should be taken between 15 minutes to 30 minutes after loading the substrates to achieve best sensitivity and dynamic range.

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