I ordered a 0.2 µmole scale oligo. Does this mean that I will receive 0.2 µmole of product?
The scale of synthesis represents the µmole of starting material for the first base of synthesis. The overall µmole yield of full-length oligonucleotide depends on the average coupling efficiency of each base and on the number of couplings (length of oligo). Sigma quotes a coupling efficiency of 98-99%. Yields of full-length oligo can vary considerably based on length and type of purification requested. Sigma has set standard minimum yield guarantees based on the synthesis and purification of your oligo. The minimum guaranteed yields are based on absorbance at 260 nm (optical density units or O.D.s) rather than µmole amounts.
