I know which segments of my sequence are transmembrane. How can I directly compare them with those determined by MPEx?
• Choose ‘Mark/Measure’ in the ‘Mark/Measure User Segments’ submenu under the ‘MPExToolbox’ menu. The ‘Mark/Measure Segments’ window will be activated. • Enter the Start and End positions of a desired segment. Press the ‘Mark’ button. • Alternatively, once the ‘Mark Helix Segs’ window is activated, click and drag the mouse within the hydropathy plot. The Start and End positions will be displayed automatically. Press the ‘Mark’ button. • A rust-colored bar appears, indicating the marked section. The free energy values displayed in the Mark/Measure Segments window and the user marked segments information in the Results window correspond to the currently selected Wimley-White scale from Hydropathy Analysis. To remove a single marked helix: • Choose ‘Mark/Measure’ in the ‘Mark/Measure User Segments’ submenu under the ‘MPExToolbox’ menu. The ‘Mark/Measure Segments’ window will be activated. • Select the marked segment you would like to remove from the list at the bottom of the window. Press
