I have tried Captiva plates for Plasma Protein Precipitation Filtration and I am still not yielding a clear filtrate. What’s the problem?
Cloudy filtrates are generally the result of the following: Using too much plasma . The filter capacity of Captiva it good up to 200 µL of plasma. Using an improper ratio of Plasma to reagent: Maintain a ration of 1 : 4 (Plasma to Reagent) Using an improper precipitation reagent: Our Recommendation is to use acetonitrile or methanol (acetonitrile works best). Some other reagents may not completely precipitate the proteins. If proteins are not precipitated before filtration they will pass through the filter. Also some reagents will require a few minutes to completely precipitate the proteins. Applying plasma directly to the Captiva plate before applying the reagent will result in the plasma leaching into the filter material. When you add the precipitation reagent all of the plasma may not be precipitated, resulting in a cloudy filtrate. Applying too much vacuum during filtration. The recommended vacuum for filtration with Captiva is < 5 in. Hg. Applying > 5 in. Hg might cause the precip
Related Questions
- I have tried Captiva plates for Plasma Protein Precipitation Filtration and I am still not yielding a clear filtrate. What’s the problem?
- What is the maximum plasma or serum sample recommended for use on Captiva 96-well 0.45 µm Protein Precipitation Plates?
- Are Captiva filters stable with all types of Protein precipitation reagents?
