I have successfully used Plasmocin before on mammalian cells, but can you tell me whether it is a suitable antibiotic for use with insect cells ?
Plasmocin can be used to clear mycoplasma infection in insect cells. The conditions are similar to those for mammalian cells, that is 25 g/ml for 2 weeks. The concentration of the antibiotic can be increased or reduced by 50% (12.5 g/ml to 37.5 g/ml) depending on the severity of mycoplasma contamination and the sensitivity of the cell line to Plasmocin.