I have selected the correct size UV-Cuvettes for my spectrophotometer, but still am not getting appropriate results. Why?
If you are using the appropriate window height (z-dimension) ultra-micro UV-Cuvette for your spectrophotometer, and are not getting appropriate results, it is likely due to one of two different problems. • Extremely dilute samples. Because the proprietary plastic has a higher absorbance than quartz, extremely dilute samples may not differ substantially from background, so you may have no other choice than to use a quartz cuvette. • Light beam width. Some spectrophotomers have abnormally wide light beams that can be refracted by the side walls of the cuvette. When using ultra-micro sized samples, these spectrophotometers require the use of black-masked quartz cuvettes to overcome this deficiency.
