I have followed the protocol and my cells are not growing, what is wrong?
Try increasing the number of cells during inoculation, adding serum at a 5% concentration to the medium compartment (for serum based applications), and verifying that the cells are in log phase when collected for inoculation. Contact the distributor or Wilson Wolf Manufacturing for assistance. An important aspect of troubleshooting your problem is specific information about the viability and the number of cells in the CELLine. Please have that information available.
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- I have followed the protocol and my cells are not growing, what is wrong?