I have early passage cells growing, can I swap them directly into the CELLnTEC medium?
No, cells grown in one medium are adapted to this medium and therefore it is necessary to be weaned away from the old medium to the new medium. This is best done in a step-wise decreasing dilution of the old medium over a period of one to four weeks, depending on the severity of the transition and the proliferation rate of the cells. For a four week approach this can be done with the following dilutions (new medium / old medium): 20 / 80%, 40 / 60%, 50 / 50%, 60 / 40%, 80 / 20% and 100 % new medium. For a two week approach the following dilutions can be used (new medium / old medium): 25 / 75%, 50 / 50 %, 75 / 25% and 100% new medium. For a one week approach the following dilutions can be used (new medium / old medium): 25 / 75%, 50 / 50 %, 75 / 25% and 100% new medium, in this case the change is made much quicker than in the two week approach and additional you should use a higher seeding density (10 to 20% more than normal) to be sure that enough cells will attach and survive to have
