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I have an Ab that is shown by the manufacturer to be positive by Western blot. Is it necessary to test it with the ProteoScan Assay Validation Array?

April 26, 2017AB assay blot manufacturer necessary positive proteoscan Test Western

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I have an Ab that is shown by the manufacturer to be positive by Western blot. Is it necessary to test it with the ProteoScan Assay Validation Array?

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We highly recommend that you do. We have tested several antibodies shown by the provider to be highly reactive by Western blot that completely failed to give any response with the array or even with lysate from cell lines. Using the ProteoScan Assay Validation Array can save you the expense, frustration and time associated with such reagents.