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I followed your protocol exactly to conjugate my antibody to the eFluor® Nanocrystals, but I see no signal in my application. What should I do?

April 26, 2017Antibody application conjugate followed nanocrystals Protocol signal

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I followed your protocol exactly to conjugate my antibody to the eFluor® Nanocrystals, but I see no signal in my application. What should I do?

1 Answer

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Certain antibodies may perform better using one chemistry over the other. For example, if an amine group is present in the antigen binding site, conjugation with the Amine-Reactive conjugation kit to this amine may block binding of the antibody to its epitope. Alternatively, if the disulfide linkages in your antibody are blocked or protected, the Sulfhydryl-Reactive kit will not work because conjugation to the eFluor® Nanocrystals will not occur. In either case, the best solution is to try the alternative kit chemistry. Please see the section(s) below concerning the application you are performing for additional troubleshooting assistance.