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I do not see apparent plaques or CPE after the initial transfection. Did I do anything wrong?

April 26, 2017apparent CPE initial plaques transfection wrong

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I do not see apparent plaques or CPE after the initial transfection. Did I do anything wrong?

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In most cases, you probably will not see any apparent plaques formed or drastic CPE even at 10 days after the transfection. We have never failed to generate viruses even though we do not observe plaques or CPE. Whether you see plaques or not probably depends on the transfection efficiency, which only affects the initial viral titer. On the other hand, if you use AdTrack derivatives, you should observe some comet-like clusters under fluorescence microscope after 5 to 7 days of transfection.