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I currently have columns in solvent “A” and wish to change to solvent “B”?

April 26, 2017columns Currently solvent wish

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I currently have columns in solvent “A” and wish to change to solvent “B”?

1 Answer

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Generally, one can switch directly from one solvent to another at 0.1 – 0.2 ml/minute (see your column care and use manual) if the two solvents are miscible. If the solvents are not miscible, an intermediate solvent (which both solvents are miscible in) will have to be used.