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I cannot make electrocompetent cells, and I cannot get the pellet into solution when preparing the cells-am I using the wrong Falcon tubes?

April 26, 2017cells electrocompetent Falcon Pellet preparing solution tubes wrong

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I cannot make electrocompetent cells, and I cannot get the pellet into solution when preparing the cells-am I using the wrong Falcon tubes?

1 Answer

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You are probably using the wrong tubes-we use ROUND-BOTTOM 14 ml polypropylene Falcon tubes (cat. number 35-2059, Becton-Dickinson) for making competent cells. If you use CONICAL tubes you will have a hard time getting the pellet into solution.