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I cannot detect any biological activity after transfecting my clone. What do I do?

April 26, 2017activity biological clone detect

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I cannot detect any biological activity after transfecting my clone. What do I do?

1 Answer

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Lack of activity can occur for a wide variety of causes. First, be sure that the preparation of DNA that you are working with is of the expected concentration and is not a purified contaminant (from your lab or from OriGene). Secondly, check to see if your protein is being expressed in your cell type by Western blot. If it is not, next check for expression of the mRNA transcript by RT-PCR. These are very different problems with very different solutions. Once you know the source of the problem, please contact our technical support scientists for assistance at 888-267-4436 (USA) or techsupport@origene.com.