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I can get good EPSPs immediately after putting the slice onto the probe, but later, the response goes away. How can I maintain a steady baseline?

April 26, 2017away epsps good immediately later probe putting Response slice

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I can get good EPSPs immediately after putting the slice onto the probe, but later, the response goes away. How can I maintain a steady baseline?

1 Answer

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The major factors involved in maintaining a steady baseline are controlling stable temperatures (incubator, baths, ACSF, humidified oxygen), correct airflow, and a stable ACSF level within the probe.