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I am using the consensus peptide described by Schatz (1993) but the levels of biotinylation are poor. How can I improve this?

April 26, 2017biotinylation consensus levels peptide poor schatz

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I am using the consensus peptide described by Schatz (1993) but the levels of biotinylation are poor. How can I improve this?

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The consensus sequence is, in effect, an average of the efficient and inefficient peptide sequences. The most efficient sequence is #85. (G L N D I F E A Q K I E W H E) You need to convert over to this.