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I am trying to create primers for a list of rs numbers, and am looking for 50 bp of flanking sequence for these SNPs. Why does dbSNP show only 25bp of flanking sequence for these SNPs?

April 26, 2017BP create flanking list Numbers Primers Rs

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I am trying to create primers for a list of rs numbers, and am looking for 50 bp of flanking sequence for these SNPs. Why does dbSNP show only 25bp of flanking sequence for these SNPs?

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In general, dbSNP does not “chop” submitted sequence to only 25 bases of flanking sequence. 25 bases of flanking sequence are the minimum number dbSNP requires a submitter to provide when they submit a SNP assay to us. If a submitter only submitted 25bp of flanking sequence to dbSNP for the SNPs you are interested in, you could contact the submitter directly to see if they may be able to provide you with more flanking sequence.