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I am submitting a single template that will be sequenced with several primers. Do I need to send 10 ul of template in individual tubes for each primer?

April 26, 2017individual Primers send sequenced Template tubes UL

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I am submitting a single template that will be sequenced with several primers. Do I need to send 10 ul of template in individual tubes for each primer?

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A. No, there is no need to send 10 ul of template in individual tubes for each primer. Instead, please send one tube of template containing the following volume: [# primers X (2 ul of template/primer)] X 1.5 = volume of template to submit.