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I am only using 50 µl (total) reagent + samples per well. Why should I change the pad before the last washing step?

April 26, 2017pad Reagent samples step total washing

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I am only using 50 µl (total) reagent + samples per well. Why should I change the pad before the last washing step?

1 Answer

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• We have observed that changing the pad helps reduce the background signal. It is not mandatory – but will help if you are pushing the detection limits.