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I am getting a different retention time for the peaks in the Resolution Test Mixture on different systems. What impact does this have on the integrity of my data?

April 26, 2017different impact mixture PEAKS resolution retention SYSTEMS Test time

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I am getting a different retention time for the peaks in the Resolution Test Mixture on different systems. What impact does this have on the integrity of my data?

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[top] No impact at all. There will certainly be mobile phase preparation differences, aging of columns, etc. The HSQ kit instructions call for adjustment of the mobile phase to meet system suitability, which for example in our C of A, calls to adjust the mobile phase to achieve a 4-5 minute retention time window. Keep in mind that the C o A merely confirms that the column was tested, and met specs when it left our facility.