I already use Mascot, SEQUEST, OMSSA, X!Tandem, etc. why should I be interested in spectral libraries?
Spectral library searching uses an algorithm that can take advantage of measured peak heights (empirical data). The spectral libraries are also smaller than an in silico digest of a whole proteome sequence file. These factors may make spectral library searching more sensitive and much faster than traditional sequence searching for your particular applications. Mass spectral library searching has also been around longer than proteomics and is the “gold standard” for interpreting unknown spectra in other fields of mass spectrometry (e.g., GC-MS); only recently has enough data been compiled and analyzed to generate reference libraries for peptides.
Related Questions
- I have GC/MS MSD ChemStation mass spectral libraries that I want to convert for use in the NIST MS Search Program; how do I do this?
- Am I allowed to distribute samples Ive made of any of Omnispheres Factory sound libraries?
- What MS spectral libraries/ databases are available for the GC/MS MSD ChemStation?
