How would i use PCR to obtain lots of DNA fragments to perform tests on?
The Polymerase Chain Reaction allows the amplification of small lengths of DNA left pretty much anywhere, so that there is an amount which is suitable for the next stage of genetic fingerprinting. Firstly the section is isolated and heated to 95 degrees celsius. This is to denature the strand to allow the next stage to take place, by breaking the hydrogen bonds between the bases. Complimentary primers are attached to the Single-Stranded DNA (ssDNA) molecules to where you want to start copying. Then it is cooled to 40 degrees Celsius to allow the primers to bond fully to the ssDNA. Then the solution is heated again to 70 degrees Celsius in a solution of free complimentary DNA nucleotides are attached to the ssDNA by the enzyme Taq Polymerase (which is more thermally-stable than eukaryote polymerase), starting at the primers, so you double the amount of DNA. These steps are repeated as many times as you want until you have the amount of DNA you’re after. In order to identify somebody by
