How will the Amine-Reactive kit affect the function of my protein?
The Amine-Reactive kit will modify available primary amine groups on your protein and then conjugate to the eFluor® Nanocrystal surface through those modifications. If the active portion of your biomolecule is known to contain primary amine functionalities, conjugation of the eFluor® Nanocrystals to these amines could block activity of your biomolecule. In this instance, you may achieve better results with the Sulfhydryl-Reactive eFluor® Nanocrystal kit.
