How were the proteins in the database matched to proteins in the PDB?
Each LOCATE protein was BLASTed against the PDB database. Proteins were then assigned a score between 0 and 5 based on the summed score of 3 parameters: E-value, coverage, and sequence identity. If the E-value was less than 10e-10, a hit was given 1 point; if the E-value was less than 10e-50, that hit was given 2 points. If the coverage of the alignment was 80% or greater, the hit was given 2 points. If the percent identity of the alignment was 98% or greater, the hit was given 1 point. Only hits that scored 4 points or more were mapped to a LOCATE protein.
