How were the genomic libraries made?
The BDGP Drosophila melanogaster BAC library was prepared by Kazutoyo Osoegawa and Aaron Mammoser in Pieter de Jong’s laboratory in the Department of Cancer Genetics at the Roswell Park Cancer Institute in Buffalo, NY. The library is named “RPCI-98” and was constructed by partial EcoRI digestion of Drosophila DNA provided by the BDGP from the isogenic strain y2; cn bw sp, the same strain used for the P1 and cDNA libraries. The construction of the RPCI-98 BAC library is described at http://www.chori.org/bacpac/98framedromel.htm. The construction of the DrosBAC library is described at http://www.hgmp.mrc.ac.uk/Biology/descriptions/dros_bac.html An explanation of the construction of the bacteriophage P1 library, can be found on the Construction of the P1-based framework page.
