How well does the NanoSight technology cope with polydispersity?
For polydisperse samples, the NanoSight system exhibits significant advantages over PCS in that the size distribution profile obtained is based on the separate but simultaneous analysis of each and every particle seen during the analysis period. This is in stark contrast to the data obtained by PCS in which a large ensemble of particles are measured and from which an intensity weighted (z-average) is obtained. The NanoSight system can distinguish between a bimodal system if the particles are 50% different in size (for example 80 nm and 120 nm particles).
