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How transformants are selected when using pSpark DNA cloning systems?

April 26, 2017cloning dna pspark Selected SYSTEMS transformants

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How transformants are selected when using pSpark DNA cloning systems?

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A6. All pSpark DNA cloning vectors have ampicillin resistance for selection of transformants and one vector has dual ampicillin/kanamycin antibiotic resistance. All inserts can be amplified by PCR with pUC/M13 forward and reverse primers and in vitro transcribed with either T7 or SP6 RNA polymerases. Blue white screening is possible in all vectors except pSpark DNA cloning systems IV and V that are transcription free.