How to sequence through difficult structure regions?
Difficult structure region includes repetitive DNA, homopolymer region (poly A, T), GC rich template (>70%), and secondary structure (inverted repeats and palindromes). They cause drops in signal intensity and noisy data immediately after the region. Sequencing the complimentary strand helps to elucidate the ambiguous data after a difficult sequencing region. Order us to use dGTP kit might improve the difficult region sequencing, although overall sequencing quality is compromised.
