How to remove and avoid air bubbles in channels?
If you culture the cells over a longer time some air bubbles can occur inside the channel. Trapped bubbles can be removed from the channel by inclining the µ-Slide and carefully knocking at one edge. To avoid emerging bubbles, put your media and the µ-Slide in a non gas-tight container and put both into your incubator the day before use. This will lead to equilibration of dissolved gasses.