How to prepare nutrient agar plates?
Prepare the nutrients such as chicken broth or beef broth and boil it with some mushroom. Let it cool off to the room temperature and filter it to be clear. Add water to bring it to 800ml to 1000 ml. Add 7 to 10 grams of agar and stir it to make an emulsion. • If you have purchased a kit with a test tube of agar powder, it contains about 7 grams of agar. Use all of that for 800 mL up to 100 mL nutrients. • If the agar comes in a glass bottle, it is 16 grams. Use half of that for 800 mL nutrients. Reheat the emotion while stirring until it boils for about one minute. Your nutrient agar is now ready. Let it cool to about 45C. At this time you can pour the nutrient agar solution in petri-dishes and place the lid. The amount of nutrient agar in each plate must be enough to cover the bottom of the plate. Agar will solidify in plates. Note that boiling does not kill all the bacteria and your agar and your plates may not be sterile at this time. If you have access to an autoclave, place your
