How to maintain the low temperature during the shearing?
There are 3 possibilities: 1. Automatic temperature control A refrigerated circulation bath can be used to guarantee the automatic temperature control of the water bath during the whole sonication process. 2. Manual temperature control Before starting the first round of sonication a “pre-cooling” of the Bioruptor®’s tank with crushed ice during 15 minutes is advised. This will allowed to avoid too quick water heating due to thermal inertia (the tank and the ultrasound generating elements are generally stored at room temperature). Then cold water should be added to the crushed ice in the waterbath up to the indicated level (sticker). We suggest you to keep a stock of water at 4°C (fridge or cold room). The crushed ice floating in the water should not exceed 0.5cm and the total water level (water and ice) should be exactly at the indicated water level. At the end of a typical sonication time (10 minutes, cycles of 30 seconds “ON” & 30 seconds “OFF”), the temperature in the water bath sho
