How to develop SSRs in species with no sequence information? Is there any alternate strategy available for SSR enrichment?
Development of a microsatellite marker system for a new species requires isolation, cloning, sequencing and characterization of microsatellite loci. Several procedures are available for the enrichment of microsatellites in genomic library like use of anchored repeat primers or probes to improve the efficiency of microsatellite isolation The development of microsatellite markers through these laboratory based screening of SSR libraries is highly time consuming and expensive. An alternate strategy is to cross amplify SSRs across species or genera followed by construction of enriched library and subsequent sequencing and development of primers. This strategy was used to develop SSRs in Casuarina equisetifolia.
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