How to design primer and probe for Real-Time PCR?
A summary of the primer and probe design guidelines is shown in Table 1. Even though no probe is required for SYBR Green I dye detection, it is still a good idea to use software to select a primer and probe set when designing a SYBR Green I assay. Although no probe will be used, the primers will meet all the required criteria and if, in the future, there is the need to convert the assay to TaqMan assay chemistry to obtain higher specificity, the probe can immediately be found in the original software document.
